A simple assay to determine the functionality of Cre or FLP recombination targets in genomic manipulation constructs |
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| Authors: | F Buchholz PO Angrand AF Stewart |
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| Affiliation: | European Molecular Biology Laboratory, Gene Expression Program, Heidelberg, Germany. |
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| Abstract: | We report the construction of two Escherichia coli strains (294-Cre and 294-FLP) which express either Cre- or FLP-recombinase. Plasmids containing authentic recognition targets for either recombinase (loxPs or FRTs) are recombined when propagated in the appropriate strain. 294-Cre and 294-FLP thus provide a simple test for the recombination competence of constructs that are designed for use in Cre- or FLP-mediated genomic manipulations. |
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