| 淋巴囊肿病病毒主要衣壳蛋白基因片段原核载体的构建及表达 |
| |
| 引用本文： | 吴兴安,胡刚,李继业,刘勇,张芳琳,于澜,白文涛,孙修勤,徐志凯. 淋巴囊肿病病毒主要衣壳蛋白基因片段原核载体的构建及表达[J]. 高技术通讯, 2003, 13(6): 78-80,65 |
| |
| 作者姓名： | 吴兴安 胡刚 李继业 刘勇 张芳琳 于澜 白文涛 孙修勤 徐志凯 |
| |
| 作者单位： | 1. 第四军医大学生物学教研室,西安,710032
2. 国家海洋局第一海洋研究所,青岛,266061 |
| |
| 基金项目： | 863计划(2001AA622030)资助项目。 |
| |
| 摘 要： | 淋巴囊肿病病毒感染牙鲆鳃细胞系FG-9307，提取细胞总RNA，采用RT-PCR法成功获得了淋巴囊肿病病毒主要衣壳蛋白0．6kb基因片段。构建其原核表达载体，IPTG诱导表达。结果表明，该融合蛋白分子量约48kD，可与抗LCDV多克隆血清特异反应。为LCDV基因工程疫苗的研制奠定了实验基础。
|
| 关 键 词： | 淋巴囊肿病病毒 主要衣壳蛋白 基因片段 原核载体 虹彩病毒 诱导表达 |
Construction and Experession of Recombinant Prokaryotic Vector Containing Lymphocystis Disease Virus MCP Gene |
| |
| Wu Xingan,Hu Gang,Li Jiye,Lun Yong,Zhang Fanglin,Yu Lan,Bai Wentao,Sun Xuqin,Xu Zhikai. Construction and Experession of Recombinant Prokaryotic Vector Containing Lymphocystis Disease Virus MCP Gene[J]. High Technology Letters, 2003, 13(6): 78-80,65 |
| |
| Authors: | Wu Xingan Hu Gang Li Jiye Lun Yong Zhang Fanglin Yu Lan Bai Wentao Sun Xuqin Xu Zhikai |
| |
| Abstract: | Paralichthys olivaceus gilled cells FG-9307 was infected by lymphocystis disease virus. The total cell RNA was extracted from it by using Trizol Reagent. LCDV major capsid protein 0.6kb gene fragment was obtained by RT-PCR. Then the prokaryotic expression vector pGEX-4Tl-LCDV0. 6 was constructed.. After inducing expressed the fusion protein by IPTG, Western blotting was used to identify the activity of the protein. The results showed that the fusion protein was about 48 kDa and it had special combination activity with anti-LCDV polyclonal serum. It established foundation to research the genetic engineering vaccine of LCDV. |
| |
| Keywords: | Lymphocystis disease virus Sequencing Majoy capsid protein Prokaryotic expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
