Trypsinization-dependent cell labeling with fluorescent nanoparticles |
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| Authors: | Tetiana Serdiuk Sergei Alekseev Vladimir Lysenko Valeriy Skryshevsky Alain Gélo?n |
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| Affiliation: | 1.University of Lyon, CarMeN Laboratory, INSA de Lyon, UMR INSERM 1060, Lyon, France;2.Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64, Volodymyrska St., 01601 Kyiv, Ukraine;3.Chemistry Faculty, Taras Shevchenko National University of Kyiv, 64, Volodymyrska St., 01601 Kyiv, Ukraine;4.University of Lyon, Nanotechnology Institute of Lyon (INL), UMR 5270, CNRS, INSA Lyon, Villeurbanne F-69621, France |
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| Abstract: | Trypsin is often used to detach adhered cell subculture from a substrate. However, the proteolytic activity of trypsin may harm cells by cleaving the cell membrane proteins. The present study shows that cellular uptake of fluorescent nanoparticles is remarkably increased within 24 h after trypsinization. These results highlight the trypsin-induced protein digestion, provoking leaky cell plasma membrane which leads to the strongly enhanced cellular uptake of the nanoparticles. To prevent this effect, one should expose cells to the nanoparticle (NP)-based fluorescent labels at least 48 h after trypsinization. |
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| Keywords: | Fluorescent nanoparticles Cell labeling Trypsin |
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