Production of Structured Phosphatidylcholine with High Content of DHA/EPA by Immobilized Phospholipase A1-Catalyzed Transesterification |
| |
| Authors: | Xiang Li Jia-Feng Chen Bo Yang Dao-Ming Li Yong-Hua Wang Wei-Fei Wang |
| |
| Affiliation: | 1.College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China; E-Mails: (X.L.); (J.-F.C.); (D.-M.L.);2.School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China; E-Mail: |
| |
| Abstract: | This paper presents the synthesis of structured phosphatidylcholine (PC) enriched with docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) by transesterification of DHA/EPA-rich ethyl esters with PC using immobilized phospholipsase A1 (PLA1) in solvent-free medium. Firstly, liquid PLA1 was immobilized on resin D380, and it was found that a pH of 5 and a support/PLA1 ratio (w/v) of 1:3 were the best conditions for the adsorption. Secondly, the immobilized PLA1 was used to catalyze transesterification of PC and DHA/EPA-rich ethyl esters. The maximal incorporation of DHA and EPA achieved was 30.7% for 24 h of reaction at 55 °C using a substrate mass ratio (PC/ethyl esters) of 1:6, an immobilized PLA1 loading of 15% and water dosage of 1.25%. Then the reaction mixture was analyzed by 31P nuclear magnetic resonance (NMR). The composition of reaction product included 16.5% PC, 26.3% 2-diacyl-sn-glycero-3-lysophosphatidylcholine (1-LPC), 31.4% 1-diacyl-sn-glycero-3-lysophosphatidylcholine (2-LPC), and 25.8% sn-glycerol-3-phosphatidylcholine (GPC). |
| |
| Keywords: | immobilized phospholipsase A1 structured phosphatidylcholine docosahexaenoic eicosapentaenoic |
|
|
