| 大孔树脂柱层析法纯化蕨菜总黄酮的工艺条件研究 |
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| 引用本文： | 陈乃富,张莉,韩邦兴,谷仿丽,何永松.大孔树脂柱层析法纯化蕨菜总黄酮的工艺条件研究[J].食品与发酵工业,2007,33(4):141-144. |
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| 作者姓名： | 陈乃富 张莉 韩邦兴 谷仿丽 何永松 |
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| 作者单位： | 皖西学院化学与生命科学系,安徽六安,237012 |
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| 基金项目： | 安微高校省级自然科学研究重点项目 |
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| 摘 要： | 从最适样品浓度、吸附速率、pH、上样量、乙醇洗脱液浓度、乙醇洗脱速率、乙醇洗脱用量等方面对AB-8大孔树脂纯化蕨菜总黄酮的工艺条件进行了研究。结果表明:其最适宜工艺条件为黄酮水溶液浓度0.3mg/mL、pH为2、上样量为5mg黄酮/g树脂,以0.5～1mL/min吸附速率进行吸附,4倍床体积的体积分数70%乙醇以0.5～1ml/min的流速进行洗脱效果最佳。未经大孔树脂纯化的蕨菜粗黄酮粉中黄酮含量是18.4%,经AB-8大孔树脂柱层析法纯化后的蕨菜总黄酮可达63.36%,纯度提高约3.4倍,此种方法纯化后得精制黄酮粉相对原粗黄酮粉的得率为18.0%。
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| 关 键 词： | 蕨菜 大孔吸附树脂 总黄酮 纯化 |
| 修稿时间： | 01 30 2007 12:00AM |
Study on the Technologic Conditions of Purifying Total Pteridium aquilinum Flavonoids by AB-8 Macro-resin Absorption |
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| Chen Naifu,Zhang Li,Han Bangxin,Gu Fangli,He Yongsong.Study on the Technologic Conditions of Purifying Total Pteridium aquilinum Flavonoids by AB-8 Macro-resin Absorption[J].Food and Fermentation Industries,2007,33(4):141-144. |
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| Authors: | Chen Naifu Zhang Li Han Bangxin Gu Fangli He Yongsong |
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| Affiliation: | Department of Chemistry and Life Science, West of Anhui University, Luan 237012 ,China |
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| Abstract: | Using AB-8 macro-resin as absorption,the technical conditions of purifying crude flavonoid powder from Pteridium aquilinum were studied in 5 aspects,which included the best drug concentration、adsorption rate、pH、adsorption ratio,etc.The best conditions were:soluble flavonoid content 0.3mg/ml,pH2,5mg flavonoids /g resin,speed at 0.5~1 ml/min.best elution condition was :4BV 70%(mL/mL)alcohol at speed 0.5~1 m/min.The total flavones of Pteridium aquilinum was 18.4% without purification.After being purified,the total flavones can be reached to 63.36%,3.4 times more than unpurified.The ratio of refined flavanoid powder to the crude flavanoids powder was 18.0%. |
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| Keywords: | Pteridium aquilinum adsorption resin total flavones purification |
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