| 流式细胞术在检测大肠埃希氏菌抗生素后效应中的应用 |
| |
| 引用本文： | 王珊,廖杰.流式细胞术在检测大肠埃希氏菌抗生素后效应中的应用[J].现代科学仪器,2006,16(2):59-61. |
| |
| 作者姓名： | 王珊 廖杰 |
| |
| 作者单位： | 解放军总医院医学实验测试中心,北京,100853 |
| |
| 摘 要： | 目的：探讨一种快速、准确检测细菌抗生素后效应（PAT）的流式细胞术.方法：选用碘化丙啶作为荧光染料,应用FACS Calibur型流式细胞仪（FCM）检测大肠埃希氏菌ATCC25922.以CellQuest软件采集、储存和分析数据.结果：清除加替沙星（GTLX 4MIC）270min后,流式细胞术可同时检测到菌体FSC,FL2增大.峰值右移,荧光强度增强.本方法是一种快速、多参数、理想的PAE检测手段.
|
| 关 键 词： | 流式细胞术 大肠埃希氏菌 抗生素后效应 |
| 收稿时间： | 2005/8/24 |
| 修稿时间： | 2005年8月24日 |
Evaluation of Detection Escherichia Coli During the Postantibiotic Effect Period by Flow Cytometry |
| |
| Wang Shan,Liao Jie.Evaluation of Detection Escherichia Coli During the Postantibiotic Effect Period by Flow Cytometry[J].Modern Scientific Instruments,2006,16(2):59-61. |
| |
| Authors: | Wang Shan Liao Jie |
| |
| Abstract: | Objective: An accurate and rapid method to detect the postantibiotic effect by flow cytometry was eaplored.Method: The changes of strain sizes and DNA contents of Escherichia coli during the postantibiotic effect(PAE) period were determined by a modified FACSC Cailbur with propidium iodide as a fluorescent probe.CellQuest software was used for data acquisition,storage and analyses.Results: The changes of FSC and FL2 were determined simultaneity by flow cytometry,after Gatifloxacin(4MIC) had been for 270min.The pead was righrt shifted and the fluorescent was strengthened.Conclusion: This method is a rapid,accurate,multi-parameter and ideal for PAE. |
| |
| Keywords: | Flow cytometery escherichia coli postantibiotic effect |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
