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Female Sex Pheromone of a Carpenter Moth, Cossus insularis (Lepidoptera: Cossidae)
Authors:Xiong Chen  Kiyoshi Nakamuta  Tomoaki Nakanishi  Tadakazu Nakashima  Masahiko Tokoro  Fumiaki Mochizuki  Takehiko Fukumoto
Affiliation:(1) Department of Forest Entomology, Forestry and Forest Products Research Institute, PO Box 16, Tsukuba Norin Kenkyu Danchi-nai, Tsukuba Ibaraki, 305-8687, Japan;(2) Fruit Tree Research Institute, Tokushima Prefectural Agriculture, Forestry and Fisheries Technology Center, Nue, Katuura, Tokushima 773-4301, Japan;(3) Shin-Etsu Chemical Co., Ltd., Specialty Chemicals Research Center, 28-1, Nishifukushima, Kubiki-ku, Joetsu, Niigata 942-8601, Japan;(4) Present address: Texas Agricultural Experimental Station, Texas A & M University System, 6500 Amarillo Boulevard, Amarillo, TX 79106, USA
Abstract:This study describes the identification of a sex pheromone component of a cossid moth, Cossus insularis. Coupled gas chromatographic–electroantennographic detection (GC–EAD) analysis of solid-phase microextraction (SPME) collections of volatiles released by live female moths showed that two compounds elicited EAG responses from the antennae of male moths. These compounds were identified as (E)-3-tetradecenyl acetate (E3-14:Ac) and (Z)-3-tetradecenyl acetate (Z3-14:Ac) by mass spectral analysis and retention index comparisons with synthetic standards. The ratio of E3-14:Ac and Z3-14:Ac was 95:5 in the effluvia of a female. In field bioassays, sticky traps baited with blends of E3-14:Ac and Z3-14:Ac showed that E3-14:Ac is an essential component of the pheromone. However, the role of Z3-14:Ac is unclear, because E3-14:Ac as a single component was as attractive to male moths as blends of E3-14:Ac and Z3-14:Ac, including the 95:5 blend released by live female moths.
Keywords:Carpenterworm moth                  Cossus insularis              (E)-3-tetradecenyl acetate  (Z)-3-tetradecenyl acetate  GC–  EAD  SPME  sex pheromone
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