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Antibacterial Effects and Cell Morphological Changes in Sfaphylococcus aureus Subjected to Low Ethanol Concentrations
Authors:SONIA A. BALLESTEROS  JORGE CHIRIFE  JUAN P. BOZZINI
Affiliation:Author Ballesteros is Becaria de Perfeccionamiento de la Universidad de Buenos Aires;author Chirife is with DeDartamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, (1428) Buenos Aires, Argentina. Author Bozzini is with lnstituto Nacional de Microbiologia, Dr. Carlos G. Malbrán, Buenos Aires.
Abstract:Ethanol in low concentrations (5–10% weight) affected aerobic growth (at 37°C) of two strains of Stuphylococcus aureus. Growth inhibition in laboratory media was observed with ethanol about 6–7% weight water activities (aw) of ~0.97. This suggested that the ethanol antibacterial effect was not only from its aw lowering ability. Electron microscopy observations showed intense morphological modifications in cells in broth with 5% to 6.5% ethanol, and clearly indicated that cell wall changes of S. aureus was partly responsible for the antibacterial action of ethanol.
Keywords:Staphylococcus aureus    antibacterial    morphological    ethanol    water activity    antimicrobial
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