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Utilization of Electrospun Polystyrene Membranes as a Preliminary Step for Rapid Diagnosis |
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| Authors: | Tu?ba Is?k Nesrin Horzum Ü Hakan Y?ld?z Bo Liedberg Mustafa M Demir |
| Affiliation: | 1. Department of Materials Science and Engineering, Izmir Institute of Technology, Urla, I˙zmir, Turkey;2. Department of Engineering Sciences, Izmir Katip ?elebi University, ?i?li, I˙zmir, Turkey;3. Department of Chemistry, Izmir Institute of Technology, Urla, I˙zmir, Turkey;4. Center for Biomimetic Sensor Science, School of Materials Science and Engineering, Nanyang Technological University, Singapore |
| Abstract: | Recent advances in clinical practice drive deoxyribonucleic acid (DNA) as an important class of biomarker. Monitoring the change in their concentration suggests the initiation and/or progression of various disorders. However, low quantity of DNA biomarkers in body fluids requires a delicate isolation methodology that provides efficient separation and easy handling. This study describes a newer‐generation separation technology relying on electrospun fibers of sub‐micrometer diameter of a commodity polymer for DNA biomarkers in simulative serum. Fibrous polystyrene membranes are prepared by electrospinning and they are subjected to post‐modification with Au. The composite membranes may provide a convenient environment for the removal of bovine serum albumin (BSA) from BSA and DNA mixtures. The eluent can be used as an efficient tool for detection of DNA biomarkers associated with diagnosis of numerous life‐threatening diseases.  |
| Keywords: | adsorption bioseparation BSA DNA biomarkers electrospinning |