| 营养因素对乳酸乳球菌DL-203生长及其Nisin生物合成的影响 |
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| 引用本文： | 焦中高, 陈海昌, 刘茵. 营养因素对乳酸乳球菌DL-203生长及其Nisin生物合成的影响[J]. 食品工业科技, 2003, (11): 22-24. DOI: 10.13386/j.issn1002-0306.2003.11.007 |
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| 作者姓名： | 焦中高 陈海昌 刘茵 |
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| 作者单位： | 1. 中国农业科学院郑州果树研究所,郑州,450009
2. 大连轻工业学院食品与生物工程系,大连,116001 |
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| 摘 要： | 对Nisin高产菌株乳酸乳球菌DL—203的营养要求及其 对Nisin生物合成的影响进行了研究。结果表明,乳链菌 肽的生物合成与DL-203的生长呈极显著正相关,乳酸 乳球菌DL—203生长和合成Nisin的最佳碳源和氮源分 别为蔗糖和大豆蛋白胨,其适宜浓度均为15～20g/L。磷 酸盐对乳酸乳球菌DL—203生长及其Nisin生物合成具 有极大的促进作用,其中以Na2HPO4效果最好,其浓度 在224mmol/L以下时对菌体生长和Nisin生物合成具有 较大的促进作用。
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| 关 键 词： | 乳酸乳球菌 乳链菌肽 生物合成 营养要求 |
| 文章编号： | 1002-0306(2003)11-0022-03 |
| 修稿时间： | 2003-03-24 |
Influence of nutrient factors on the growth of Lactoccus latis subsp. Lactis DL-203 and its biosynthesis of Nisin |
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| Jiao Zhonggao et al. Influence of nutrient factors on the growth of Lactoccus latis subsp. Lactis DL-203 and its biosynthesis of Nisin[J]. Science and Technology of Food Industry, 2003, 0(11): 22-24. DOI: 10.13386/j.issn1002-0306.2003.11.007 |
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| Authors: | Jiao Zhonggao et al |
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| Affiliation: | Jiao Zhonggao et al |
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| Abstract: | ln this paper the nutrition requirements of Lactococcus lactis Subsp. lactis DL -203 and its influence on the biosynthesis of Nisin were studied. The relationship between growth of DL-203 and biosynthesis of Nisin showed their significant positive correlation.The optimum carbon source and nitrogen souce were sucrose and soybean peptone respectively, and the optimum concentration for each was 1.5 ~2.0% (w/v). Phosphate could promote the growth of DL-203 and its biosynthesis of Nisin significantly, and Na2HPO4 showed the best effect, it showed better promotive effects on the growth of DL-203 and its biosynthesis of Nisin when the concentration was below 224 mmol/L. |
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| Keywords: | Lactococcus lactis subsp. Lactis Nisin biosynthesis nutrition requirement |
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