| 便携式MicroRNA快速检测系统 |
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| 引用本文： | 王燕飞,余东升,陈海燕,张战盈,方蔚恺,杨喆,陆泽橼,李艳蕾,纪玉峰,关一夫,徐赤东,江海河.便携式MicroRNA快速检测系统[J].光学精密工程,2018,26(3):541-547. |
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| 作者姓名： | 王燕飞 余东升 陈海燕 张战盈 方蔚恺 杨喆 陆泽橼 李艳蕾 纪玉峰 关一夫 徐赤东 江海河 |
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| 作者单位： | 1. 中国科学院 合肥物质科学研究院 医学物理与技术中心, 安徽 合肥 230031;2. 中国科学技术大学, 安徽 合肥 230026;3. 医学物理与技术安徽省重点实验室, 安徽 合肥 230031;4. 中国医科大学, 辽宁 沈阳 110001 |
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| 基金项目： | 国家自然科学基金资助项目（No.81371896） |
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| 摘 要： | MicroRNA(miRNA)与疾病的发生和发展密切相关,可作为疾病诊断标志物。常规检测miRNA的方法耗时较长且操作复杂,本文设计了一种便携式miRNA快速检测系统。该系统采用光电检测技术检测滚环扩增后的标志物miRNA受激发出的荧光强度,对采集到的荧光数据进行分析,得出miRNA的变化情况。实验得出便携式miRNA快速检测系统在试剂浓度为0.1~1μmol时,荧光强度线性偏倚不超过±5%,系统重复性大于95%。通过对冠心病检测标志物miR-499进行滚环扩增实验,结果表明设计的便携式miRNA快速检测系统能够快速有效地检测miRNA。
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| 关 键 词： | 光电检测 荧光检测 弱光探测 miRNA 滚环扩增 |
| 收稿时间： | 2017-07-10 |
Portable rapid microRNA detection system |
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| WANG Yan-fei,YU Dong-sheng,CHEN Hai-yan,ZHANG Zhan-ying,FANG Wei-kai,YANG Zhe,LU Ze-yuan,LI Yan-lei,JI Yu-feng,GUAN Yi-fu,XU Chi-dong,JIANG Hai-he.Portable rapid microRNA detection system[J].Optics and Precision Engineering,2018,26(3):541-547. |
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| Authors: | WANG Yan-fei YU Dong-sheng CHEN Hai-yan ZHANG Zhan-ying FANG Wei-kai YANG Zhe LU Ze-yuan LI Yan-lei JI Yu-feng GUAN Yi-fu XU Chi-dong JIANG Hai-he |
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| Affiliation: | 1. Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China;2. University of Science and Technology China, Hefei 230026, China;3. Anhui Province Key Laboratory of Medical Physics and Technology, Hefei 230031, China;4. China Medical University, Shenyang 110001, China |
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| Abstract: | MicroRNA (miRNA) is closely related to the occurrence and development of certain diseases and can be used as a diagnostic marker. Conventional methods for the detection of miRNA are time-consuming and complex. Herein, a portable rapid miRNA detection system was designed. This system records the fluorescence intensity of the marker that is amplified via rolling circle amplification using a photoelectric detection technique, and analyzes the fluorescence data to obtain the changes in miRNA. Through our experiments, we observed that for reagent concentrations in the range 0.1-1 μmol, the linearity of the fluorescence intensity detected by the miRNA fluorescence detection equipment was less than ±5%, and the repeatability was ≥ 95%. Our roll amplification experiment involving the coronary heart disease diagnostic marker, miR-499, confirmed that the portable rapid miRNA detection system can detect miRNA quickly and effectively. |
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| Keywords: | photoelectric detection fluorescence detection light detection miRNA rolling circle amplification |
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