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响应曲面法优化番茄红色素的提取条件
引用本文:温拥军,陈杰山,游玟娟,罗燕南. 响应曲面法优化番茄红色素的提取条件[J]. 中国调味品, 2011, 36(10)
作者姓名:温拥军  陈杰山  游玟娟  罗燕南
作者单位:湖南化工职业技术学院,湖南株洲,412004
摘    要:以番茄干粉为原料,采用Plackett-Burman试验设计、最陡爬坡试验结合Box-Behnken设计对番茄红色素的提取工艺进行响应曲面优化.研究结果表明;番茄红色素的最大吸收峰为472 nm,提取温度、提取时间、Ca2+浓度对番茄红色素的提取影响显著,最陡爬坡试验表明响应中心为:提取温度50℃、提取时间110 min、Ca2+ 12 mmol/L,最后用Box-Behnken响应面技术建立了关键影响提取率的二次多项式数学模型:Y-0.902+0.09X1-0.039X2+0.046X3-0.139X12-0.104X22-0.070X32+0.056X1X2+0.068X2X3,得到最佳提取工艺为:提取温度为53℃;提取时间为111 min; Ca2+的浓度为13 mmol/L.模型预测结果提取率达到92.30%,验证试验结果提取率达到92.07%.

关 键 词:番茄红色素  提取工艺  Plackett-Burman设计  最陡爬坡试验  Box-Behnken设计

Optimization of extraction process of lycopene by response surface methodology
WEN Yong-jun,CHEN Jie-shan,YOU Wen-juan,LUO Yan-nan. Optimization of extraction process of lycopene by response surface methodology[J]. China Condiment, 2011, 36(10)
Authors:WEN Yong-jun  CHEN Jie-shan  YOU Wen-juan  LUO Yan-nan
Affiliation:WEN Yong-jun,CHEN Jie-shan,YOU Wen-juan,LUO Yan-nan(Hunan Chemical Industry Vocational Technology College,Zhuzhou 412004,China)
Abstract:Plackett-Burman design(PBD),Steepest ascent experiment(SAE),and Box-Behnken design(BBD) were used to evaluate the influence of related factors on lycopene extraction.The PBD results showed that temperature,time,Ca2+ concentration have substantial influence on lycopene yield.Then SAE was also used to further optimize extraction technology.At last BBD was applied to establish the second-order polynomial equation for lycopene extraction.The results was as follows: Y=0.902+0.09X1-0.039X2+0.046X3-0.139X21-0.104X...
Keywords:extraction process  Plackett-Burman design  Steepest ascent experiment  Box-Behnken design  
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