RNA干扰Apg-2基因表达对BaF3-MIGR1和BaF3-p210细胞凋亡的影响 |
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引用本文: | 陈熙,钟梁,肖青,王欣,李春莉,冯文莉.RNA干扰Apg-2基因表达对BaF3-MIGR1和BaF3-p210细胞凋亡的影响[J].粉末涂料与涂装,2013,26(6):800-803. |
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作者姓名: | 陈熙 钟梁 肖青 王欣 李春莉 冯文莉 |
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作者单位: | 1. 重庆医科大学临床血液学教研室临床检验诊断学教育部重点实验室,重庆,400016 2. 重庆医科大学附属第一医院血液科,重庆,400016 |
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基金项目: | 高等学校博士学科点专项基金(项目编号:20050631007) |
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摘 要: | 目的探讨RNA干扰Apg-2基因表达对BaF3-MIGR1和BaF3-p210(Bp210)细胞凋亡的影响。方法取BaF3-MIGR1和Bp210、稳定抑制Apg-2表达的BaF3-MIGR1-Hspa42和Bp210-Hspa42细胞及阴性对照Bp210-HspaHK和BaF3-MIGR1-HspaHK细胞,采用瑞特染色法观察各组细胞的形态学变化;流式细胞术检测各组细胞的凋亡情况;Western blot法检测各组细胞中凋亡相关蛋白Bax和Bcl-2的表达水平。结果干扰Apg-2基因表达后,细胞出现肿胀、胞浆空泡、核染色质聚集、核碎裂,部分细胞可见凋亡小体,处于有丝分裂中期细胞明显减少。与BaF3-MIGR1和Bp210细胞相比,BaF3-MIGR1-Hspa42和Bp210-Hspa42细胞的凋亡率显著增加(P<0.05);Bcl-2蛋白的表达水平下调,Bax蛋白的表达水平无明显变化。结论干扰Apg-2基因表达可促进BaF3-MIGR1和Bp210细胞凋亡增加,其可能是通过降低抗凋亡蛋白Bcl-2的表达水平来实现的。
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关 键 词: | Apg-2基因 RNA干扰 慢性粒细胞白血病 Bcr-Abl融合基因 细胞凋亡 |
Effect of interference of Apg-2 gene expression by RNA on apoptosis of BaF3-MIGR1 and BaF3-p210 cells |
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Abstract: | Objective To investigate the effect of interference of Apg-2 gene expression by RNA on the apoptosis of BaF3-MIGR1 and BaF3-p210(Bp210) cells.Methods BaF3-MIGR1 and Bp210 cells,BaF3-MIGR1-Hspa42 and Bp210-Hspa42 cells in which Apg-2 expression was inhibited stably,as well as Bp210-HspaHK and BaF3-MIGR1HspaHK cells as negative control were observed for morphologies by Wright staining,for apoptosis by flow cytometry,and for expression of apoptosis-related Bax and Bcl-2 proteins by Western blot.Results A series of morphological changes including swelling,cytoplasmic vacuoles,chromatin aggregation,nuclear fragmentation were observed in the cells after interference of Apg-2 gene expression,and apoptotic body formation in partial cells.However,the cells at metaphase decreased significantly in quantity(P < 0.05).As compared with those of BaF3-MIGR1 and Bp210 cells,the apoptotic rates of BaF3-MiGR1-Hspa42 and Bp210-Hspa42 cells increased significantly(P < 0.05),while the Bcl-2 expression level decreased,and Bax expression level showed no significant change.Conclusion Interference of Apg-2 gene expression promoted the apoptosis of BaF3-MIGR1 and Bp210 cells through a possible mechanism of down-regulating the expression of anti-apoptotic protein Bcl-2. |
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Keywords: | Apg-2 gene RNA interference Chronic myelogenous leukemia Bcr-Abl fusion gene Cell apoptosis |
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