| 脂肪酶固定化研究和应用 |
| |
| 引用本文： | 崔娟,吴坚平,杨立荣,孙志浩.脂肪酶固定化研究和应用[J].化学反应工程与工艺,2005,21(1):43-48. |
| |
| 作者姓名： | 崔娟 吴坚平 杨立荣 孙志浩 |
| |
| 作者单位： | 浙江大学材料与化工学院生物工程研究所,浙江,杭州,310027;江南大学工业生物技术教育部重点实验室,无锡,214036 |
| |
| 基金项目： | 国家自然科学基金重点资助项目(20336010)
国家973计划资助项目(2003CB716008) |
| |
| 摘 要： | 采用硅藻土作载体，进行了脂肪酶的固定化。利用固定化酶选择性催化1-苯乙醇与乙酸乙烯酯的转酯化反应，得到R-乙酸苯乙酯，进行1-苯乙醇的拆分。实验考察了不同吸附方法固定化酶的效果，确定效果最好的固定方法为载体涂布法，并对该法的固定化条件进行了优化。制备的固定化酶的转酯比活比游离酶提高了14．3倍。固定化没有改变酶的选择性．对映体过剩值仍大于98％。初步探讨了固定化酶和游离酶的反应过程。
|
| 关 键 词： | 固定化 脂肪酶 1-苯乙醇 硅藻土 |
| 文章编号： | 1001-7631(2005)01-0043-06 |
| 修稿时间： | 2003年12月30 |
Study on the Immobilization of Lipase |
| |
| Cui Juan,Wu Jianping,Yang Lirong,Sun Zhihao.Study on the Immobilization of Lipase[J].Chemical Reaction Engineering and Technology,2005,21(1):43-48. |
| |
| Authors: | Cui Juan Wu Jianping Yang Lirong Sun Zhihao |
| |
| Abstract: | The immobilization of lipase with diatomite as the carrier was studied. Three absorption methods of lipase were selected and the factors influencing the transestification activity of immobilized lipase were investigated. The experimental results showed that the immobilized method with aggradation by vacuum-desiccation onto diatomite is better. Under the optimal conditions, the transesterification activity of immobilized lipase by the method was 14. 3 times higher than free lipase. The selectivity of reaction was not changed by immobilization. 1-Phenethyl alcohol was resolved by transesterification reaction in vinyl acetate using the immobilized lipase. |
| |
| Keywords: | immobilization lipase 1-phenethyl alcohol diatomite |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
