Biocatalytically induced formation of cupric ferrocyanide nanoparticles and their application for electrochemical and optical biosensing of glucose

The enzymatically controlled growth of cupric ferrocyanide nanoparticles in the presence of glucose oxidase, its ferricyanide electron acceptor, and copper ions is described. The biocatalytically stimulated growth of these nanoparticles on the surface of carbon-paste electrodes results in an amplified electrochemical detection of the glucose substrate. This concept can readily be expanded for monitoring a wide range of biocatalytic processes involving the ferricyanide electron acceptor.