转基因大豆Roundup Ready调控元件在食品加工过程中降解变化的研究

35S启动子可能会通过基因的水平转移插入到某一致癌基因上游，活化并导致癌症的发生。为了解转基因植物中调控元件的安全性问题，以转基因大豆Roundup Ready为实验材料，针对Roundup reaqdy转基因大豆中CaMV35S启动子及NOS终止子的序列，设计了不同长度片段的引物，通过对CaMV35S启动子和NOS终止子的PCR扩增，研究了豆腐、豆奶、豆粉3种大豆加工食品中磨浆、煮浆、调配、均质、杀菌、喷雾干燥等关键工艺对Roundup Reaqly大豆中调控元件的影响。结果表明调控原件在食品加工过程中的降解变化与其所处位置有较大关系。扩增长度相近的2个片段，包含大豆基因组。DNA序列的片段受加工过程的影响较小，在3种豆制品的所有加工过程中均能检测到。而只包含CaMV35S启动子序列的片段仅能在原料中检测到，原料经过磨浆后。片段大小降至200bp以下。NOS终止子受食品加工工艺的破坏和影响较小，在被检测食品的每一个加工过程中都能够检测到NOS终止子片段。

Degradation of non-target genes during different manufacturing processes of Roundup Ready (RR) soybean products

Regulation elements which are normally used in transgenic organisms such as CaMV35S promoter and NOS terminator have potential risk for human health due to their functions. The degradation of non target genes in the RR soybean during the manufacturing process of tofu, soymilk and soybean flour milling, cooking, blending, homogenizing, sterilizing and spray drying was investigated using a sensitive polymerase chain reaction detection system. The system used specific amplification of RR soybean promoter 35S gene and terminator NOS gene. The results showed that the degradation of CaMV35S was more related to its location than to the food processing. The fragment which included soybean genome DNA sequence was more stable than that without soybean genome DNA sequence, but the terminator NOS gene was hardly degraded during each step of the manufacturing process of RR soybean products.