Preparation of 3-D regenerated fibroin scaffolds with freeze drying method and freeze drying/foaming technique |
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Authors: | Qiang Lv QingLing Feng |
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Affiliation: | (1) Department of Materials Science and Engineering, Tsinghua University, Beijing, 100084, People’s Republic of China |
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Abstract: | Although three-dimensional fibroin scaffolds have been prepared with freeze drying method, the porosity and pore sizes still
can not satisfy the requirement of tissue engineering. In this article, fibroin porous scaffold with high porosity and > 100μm
diameter interconnected pores was firstly prepared with freeze drying method through adjusting fibroin concentration. The
morphology of different scaffolds lyophilized from different fibroin concentration was observed by SEM. A novel freeze drying
improved method, freeze drying/foaming technique, was also devised to prepare fibroin scaffolds at different fibroin concentrations.
Using the said method, the porosity and pore size of fibroin scaffolds prepared from 12% concentration were 85.8 ± 4% and
109 ± 20 μm respectively with yield strength up to 450 ± 6 KPa while the porosity and pore size of fibroin scaffolds prepared
from 8% concentration were 96.9 ± 3.6% and 120 ± 30 μm respectively with yield strength up to 30 ± 1 KPa. The freeze drying/foaming
technique produced scaffolds with a useful combination of high yield strength, interconnected pores, and pore sizes greater
than 100 μm in diameter. Through adjusting fibroin concentration and thawing time, the porosity, pore sizes and mechanical
properties could be controlled to satisfy the different requirements of tissue engineering. The results suggested that fibroin
scaffolds prepared with the above methods could be formed for utility in biomaterial application. |
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