Production of biogenic amines by lactic acid bacteria and bifidobacteria isolated from dairy products and beer |
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Authors: | Eva Lorencová Leona Buňková Dagmar Matoulková Vladimír Dráb Pavel Pleva Vlastimil Kubáň František Buňka |
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Affiliation: | 1. Department of Fat, Tenside and Cosmetics Technology, Faculty of Technology, Tomas Bata University in Zlín, nám. T. G. Masaryka 5555, 760 01?Zlín, Czech Republic;2. Research Institute of Brewing and Malting, Lipová 15, 120 44 Praha 2, Czech Republic;3. Dairy Research Institute, Ke Dvoru 12a, 160 00 Praha, Czech Republic;4. Department of Food Technology and Microbiology, Faculty of Technology, Tomas Bata University in Zlín, nám. T. G. Masaryka 5555, 760 01 Zlín, Czech Republic |
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Abstract: | The aim was to monitor production of eight biogenic amines (BAs) (histamine, tyramine (TYR), tryptamine, putrescine, cadaverine (CAD), phenylethylamine, spermine and spermidine) by selected 81 lactic acid bacteria (LAB) strains: Lactobacillus, Lactococcus, Leuconostoc, Enterococcus, Pediococcus, Tetragenococcus and Bifidobacterium. The tested LAB and bifidobacteria were isolated from dairy products and beer. The decarboxylase activity of the micro‐organisms was studied in growth medium after cultivation. The activity was monitored by HPLC after the pre‐column derivatisation with dansylchloride. Fifty LAB showed decarboxylase activity. Thirty‐one strains produced low concentrations of CAD (≤10 mg L?1). Almost 70% of beer isolates generated higher amounts of TYR (≤3000 mg L?1). Most of the tested LAB demonstrated decarboxylase activity. The above micro‐organisms can contribute to the increase of content of BAs in dairy products or beer and thereby threaten food safety and health of consumers. Production of BAs even by the representatives of some probiotic strains (Bifidobacterium and Lactobacillus rhamnosus) was detected in this research. This study has also proved that contaminating LAB can act as sources of higher amounts of CAD and TYR in beer. |
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Keywords: | Bifidobacterium biogenic amines decarboxylase activity histamine lactic acid bacteria tyramine |
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