安徽省烟草病毒种类的血清学鉴定

2007 年从安徽亳州、宣城和芜湖等地的烟草产区共采集181 个表现明显病毒病症状的病样，采用Tas-ELISA 和间 接ELISA 方法共检测112 个病样，其中分别检测宣城黄渡、宣城金坝、芜湖红杨、芜湖三元各16 个病样以及亳州陈店48个病样。结果显示，安徽宣城32 个病样中，TMV、CMV、PVY 的阳性率分别为91%、3%、50%，芜湖32 个病样中，TMV、CMV、PVY 的阳性率分别为66%、0、75%，说明宣城、芜湖烟区侵染烟草的病毒种类以TMV、PVY 为主，CMV 为零星侵染；亳州48 个病样中，TMV、CMV、PVY 的阳性率分别为100%、48%、8%，说明亳州烟区侵染烟草的病毒种类以TMV、CMV 为主，PVY 侵染发生较少。

Serological Identification of Tobacco Viruses Derived from Anhui Province

One hundred and eighty one tobacco samples with obvious virus symptoms were collected from Bozhou, Xuancheng and Wuhu of Anhui Province, 2007. The 112 samples, including 16 samples from Huangdu Xuancheng, Jinba Xuancheng, Hongyang Wuhu, Sanyuan Wuhu, respectively and 48 samples from Chendian Bozhou, were detected by Tas-ELISA and indirect ELISA methods. The results demonstrated that the positive rates of TMV, CMV and PVY of 32 samples from Xuancheng, Anhui were 91%, 3% and 50%, respectively, while those from Wuhu were 66%, 0 and 75%, respectively. It suggested that the major virus types infecting tobacco from Xuancheng and Wuhu were TMV and PVY, while CMV only occurred sporadically. The positive rates of TMV, CMV and PVY of 48 samples from Bozhou were 100%, 48% and 8%, respectively. It showed that the major virus types infecting tobacco from Xuancheng and Wuhu were TMV and CMV, and PVY infection rarely occurred.