| 重组毕赤酵母生产外源蛋白的过程控制与生理分析的研究进展 |
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| 引用本文： | 高敏杰,张许,高鹏,史仲平.重组毕赤酵母生产外源蛋白的过程控制与生理分析的研究进展[J].食品工业科技,2014(24):389-395. |
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| 作者姓名： | 高敏杰 张许 高鹏 史仲平 |
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| 作者单位： | 江南大学生物工程学院;江南大学工业生物技术教育部重点实验室 |
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| 摘 要： | 毕赤酵母是近年来应用广泛、极受青睐的一种外源蛋白表达系统。除了构建高效、稳定的毕赤酵母生产菌株外,研究不同发酵控制条件下,细胞的生理和代谢状态变化,为毕赤酵母细胞提供最优的生长和环境条件并综合考虑发酵成本是成功实现外源蛋白规模化、商业化生产的保障。本文从培养基组分、外界环境条件、细胞比生长速率、生长期甘油流加策略和诱导期甲醇流加策略等方面阐述了发酵过程对外源蛋白表达的影响,并进一步从细胞生理和代谢层面深入讨论了不同发酵过程控制条件的影响机制。
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| 关 键 词： | 毕赤酵母 过程控制 代谢分析 蛋白分泌 压力应激 |
| 收稿时间： | 2014-08-04 |
Process control and physiological analysis of heterologous protein production by recombinant Pichia pastoris |
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| GAO Min-jie,ZHANG Xu,GAO Peng,SHI Zhong-ping.Process control and physiological analysis of heterologous protein production by recombinant Pichia pastoris[J].Science and Technology of Food Industry,2014(24):389-395. |
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| Authors: | GAO Min-jie ZHANG Xu GAO Peng SHI Zhong-ping |
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| Affiliation: | 1.School of Biotechnology, Jiangnan University;2.Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University |
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| Abstract: | Pichia pastoris was currently one of the most effective and versatile systems for the expression of heterologous proteins. Besides the construction of efficient and stable recombinant Pichia pastoris, fermentation process engineering technique, mainly covering the methods of control proper fermentation conditions and envirements, that is considered as another important route for successful production of recombinant protein.The components of culture medium, environment conditions, cell specific growth rate, glycerol feeding strategies in growth period and methanol feeding strategies in induction period were summarized in this review. In additon, cell physiological and metabolic funcations in different fermentation conditions were analyzed. |
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| Keywords: | Pichia pastoris process control physiological analysis protein secretion stress response |
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