| Abstract: | The Role of Prostaglandins in Hormone-Stimulated Lipolysis in Isolated Fatty Tissues Biosynthesis of PGE from 14C labelled unsaturated fatty acids by enzyme preparations from epididymal fatty tissues of rats and rams is reported. Di-homo-γ-linolenic acid and arachidonic acid were detected in di- and triglyceride fractions of the fatty tissues of rats. The amounts found are perhaps sufficient for explaining quantitatively the synthesis of PGE in intact fatty tissues. The specificity of anti-lipolytic action of PGE homologues was studied. Prostaglandins from EFA-active fatty acids with 20 and 21 C-atoms are as active as PGE1, whereas prostaglandins from EFA-inactive fatty acids are much less active. The possible action of PGE1 on the formation and degradation of cyclic adenosine-3′,5′-monophosphate was studied with isolated enzyme system. A direct action of PGE1 could not be detected. |
