Cloning of the Escherichia coli radC gene: identification of the RadC protein |
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| Authors: | I Felzenszwalb S Boiteux J Laval |
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| Affiliation: | Departamento de Bifísica e Biometria, Universidade do Estado do Rio de Janeiro, Brasil. |
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| Abstract: | 1. The DNA sequence of the radC gene suggests an open reading frame of 297-bp. 2. To identify the gene product, radC was subcloned in an expression vector, pKK223-3 and the RadC protein identified by the maxicell method as a polypeptide of approximately 11 kDa. |
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