| Affiliation: | 1. College of Life Sciences, Xinyang Normal University, Xinyang, China;2. College of Intelligent Science and Control Engineering, Jinling Institute of Technology, Nanjing, China;3. Analysis and Testing Center, Xinyang Normal University, Xinyang, China;4. OMFS-IMPATH Research Group, Department of Biomedical Sciences, KU Leuven and Department of Oral and Maxillofacial Surgery, University Hospitals Leuven, Leuven, Belgium;5. Department of Imaging & Pathology, University of Leuven and Oral and Maxillofacial Surgery, University Hospitals Leuven, Leuven, Belgium;6. College of Life Sciences, Xinyang Normal University, Xinyang, China
State Key Lab of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, China;7. BioMatter unit - École polytechnique de Bruxelles, Université Libre de Bruxelles, Brussels, Belgium |
| Abstract: | Biphasic calcium phosphate (BCP) based materials possessed with both excellent biocompatibility and antibacterial activity show potential advantages for biomedical applications. Here, the silver-doped BCP/Alginate (AgBA) microclusters were first fabricated using the double-emulsions method. First, BCP nanoparticles were incorporated into the alginate network to form BCP/Alginate microclusters via the emulsion process. Then, silver nanoparticles (AgNPs) were in situ involved in BCP/Alginate networks to obtain the final AgBA microclusters. Transmission electron microscopy and scanning electron microscopy confirmed that BCP nanoparticles and AgNPs were uniformly distributed in AgBA microclusters. The morphology of AgBA microclusters could be regulated by adjusting emulsion power, and microclusters using the medium powder (500 W) showed a regular spherical shape. Furthermore, CCK-8 analysis identified that AgBA microclusters were cytocompatible culturing with human bone marrow-derived mesenchymal stem cells. Qualitative antibacterial tests exhibited the excellent inhibition effects of AgBA microclusters against Staphylococcus aureus (Gram-positive) and Escherichia coli. (Gram-negative). Lastly, the doxorubicin (DOX)-loaded AgBA microclusters presented adjustable loading efficiency of DOX and controllable release profiles. The cumulative release could reach 73.3% after 72 h in PBS. The above results raised a new route for antibacterial microclusters development for biomedical applications. |
