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A polyphenolic biomacromolecule prepared from a flavonoid: Catechin as degradable microparticles |
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| Authors: | Selin Sagbas Suner Subhra Mohapatra Ramesh S. Ayyala Venkat R. Brethanabotla Nurettin Sahiner |
| Affiliation: | 1. Department of Chemistry, Faculty of Sciences & Arts, and Nanoscience and Technology Research and Application Center (NANORAC), Canakkale Onsekiz Mart University Terzioglu Campus, Canakkale, Turkey Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, Florida, USA;2. Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, Florida, USA;3. Department of Ophthalmology, Morsani College of Medicine, University of South Florida Eye Institute, Tampa, Florida, USA;4. Department of Chemical & Biomedical Engineering, and Materials Science and Engineering Program, University of South Florida, Tampa, Florida, USA;5. Department of Chemistry, Faculty of Sciences & Arts, and Nanoscience and Technology Research and Application Center (NANORAC), Canakkale Onsekiz Mart University Terzioglu Campus, Canakkale, Turkey |
| Abstract: | Catechin (CAT) was crosslinked with trimethylolpropane triglycidyl ether (TMPTGE) to obtain degradable poly(CAT) particles in a single step. Spherical p(CAT) particles with tens of micrometer size range and an isoelectronic point at pH 1.2 were obtained. The hydrolytic degradation of p(CAT) particles provided sustainable and extended release with 264 mg/g CAT release within 10 days at pH 7.4. The antioxidant capacity of 55.0 ± 0.9 μg/ml gallic acid equivalent in terms of total phenol content, and 0.88 ± 0.3 μmol/g trolox equivalent were estimated for p(CAT) particles displaying strong radical scavenging capability. Blood clotting and hemolysis assays demonstrated dose-dependent blood compatibility revealing higher blood compatibility for p(CAT) particles up to 10 μg/ml concentration. The cytotoxicity results show that p(CAT) particles have almost no toxicity for CCD841 normal colon cells at 250 μg/ml concentration in 24 h incubation time giving ~97% cell viability, whereas CAT molecules only provide ~34% cell viability. |
| Keywords: | biodegradable biomaterials biopolymers catechin renewable polymers |