Fabrication and characterization of electrospun psyllium husk-based nanofibers for tissue regeneration |
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Authors: | Suruchi Poddar Piyush Sunil Agarwal Ajay Kumar Sahi Neelima Varshney Kiran Yellappa Vajanthri Sanjeev Kumar Mahto |
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Affiliation: | 1. Tissue Engineering and Biomicrofluidics Laboratory, School of Biomedical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi, India;2. Tissue Engineering and Biomicrofluidics Laboratory, School of Biomedical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi, India Department of Materials Engineering, Indian Institute of Science, Bangalore, India |
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Abstract: | The present study reports for first time the blending of psyllium husk (PH) powder/gelatin (G) in the polymer-rich composition of polyvinyl alcohol (PVA) to make an electrospinnable solution. The composite was prepared in 3 different ratios viz., 100% (wt/wt) (PVA + PH), 75% + 25% (PVA + 75PH + 25G) (wt/wt) and 50% + 50% (PVA + 50PH + 50G) (wt/wt) in 6% PVA solution. Optimum electrospinning parameters were evaluated for all the prepared blends. The fabricated nanofibers were characterized by scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared, differential scanning calorimetry, porosity percentage, and fiber orientation using ImageJ software. A qualitative in vitro degradation study at room temperature is supported by SEM images. The cellular interactions were characterized by MTT assay of NIH-3T3 fibroblast cells for 2 and 4 days with an optimum cell growth of >50% by fourth day of culture and long-term cultivation of L929-RFP cells was observed for 10 days. The nanofibers were formed in the range of 49–600 nm. PVA + 75PH + 25G when cultured with L929-RFP cells exhibited highest fluorescence intensity and thus supported cellular proliferation significantly. Based on the results obtained from various analyses, we anticipate that fabricated psyllium-based nanofiber can be used as a promising candidate for wound healing and other biomedical applications. |
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Keywords: | biomaterials biomedical applications electrospinning fibers polysaccharides |
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