Anthocyanin Determination in Black Raspberry (Rubus occidentalis) and Biological Specimens Using Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry |
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| Authors: | Qingguo Tian Robeena M Aziz Gary D Stoner Teven J Schwartz |
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| Affiliation: | Authors Tian and Schwartz are with Dept. of Food Science and Technology, The Ohio State Univ., 2015 Fyffe Rd., Columbus, Ohio 43210. Authors Aziz and Stoner are with Div. of Environmental Health Sciences, School of Public Health and Ohio State Univ. Comprehensive Cancer Center, Columbus, Ohio. Direct inquiries to author Schwartz (E-mail: ). |
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| Abstract: | ABSTRACT: The capabilities of mass spectrometry for microscale determination of anthocyanins were investigated using high-performance liquid chromatography electrospray ionization mass spectrometry (LC-ESI/ MS) and tandem mass spectrometry (MS-MS). Four anthocyanins cyanidin 3-glucoside, cyanidin 3-sambubioside, cyanidin 3–(2G?xylosylrutinoside) and cyanidin 3-rutinoside] were characterized in black raspberry samples by LC-ESI/MS-MS using both positive and negative ion analyses. Quantification of anthocyanins was conducted using ESI/MS-MS with selected reaction monitoring (SRM). Linear responses of several anthocyanins were determined during MS-MS analyses. Detection limits as low as 1 femtomol for most anthocyanins were obtained during ESI/MS-MS. Compared with other quantitative procedures such as high-performance liquid chromatography (HPLC) and ultraviolet/visible spectrophotometry, the current method provides an improved sensitive, specific technique for direct determination of intact anthocyanins. The developed methodology was successfully applied to analysis of trace levels of anthocyanins in human plasma and epithelial cells. |
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| Keywords: | anthocyanins human plasma cells quantification LC-ESI/MS MS-MS |
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