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A method for determining the mutual diffusion coefficient of molecular solutes based on surface plasmon resonance sensing |
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| Authors: | F.C.C.L. LoureiroAuthor VitaeA.G.S. Barreto NetoAuthor Vitae C.S. MoreiraAuthor Vitae A.M.N. LimaAuthor Vitae H. NeffAuthor Vitae |
| Affiliation: | a Department of Electrical Engineering, Center for Electrical Engineering and Informatics, Universidade Federal de Campina Grande, Rua Aprígio Veloso 882, 58429-900 Campina Grande, PB, Brazil b IF-PB, Department of Electromechanics, Brazil c IF-AL, Department of Electronics, Brazil d Northeast Center for Strategic Research (CETENE), Systems and Integrated Circuits Laboratory (LINCS), Recife, PE, Brazil |
| Abstract: | An experimental method, combining surface plasmon resonance sensing and microfluidics, to determine the mutual diffusion coefficient of molecular solutes, as ethanol and bovine serum albumin, is presented. Representative refractive index variations of analyte samples over time, and associated dynamic solute concentration profiles, respectively, have been employed to access molecular transport parameters. From both, Fick’s diffusion length and Taylor’s pulse dispersion methods, solute and solvent mutual diffusion coefficients for diluted ethanol and concentrated protein aqueous solutions have been obtained. Additionally, the dynamic behavior and geometry effects of molecular transport have been exploited using finite element simulations for the 3-dimensional case and confirmed experimentally. The numerical simulation also addresses the influence of temperature effects. |
| Keywords: | Microfluidic Diffusivity Surface plasmon resonance sensing Finite element simulation Molecular transport |
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