大孔树脂分离纯化女贞子中齐墩果酸工艺的研究

本文对大孔吸附树脂分离纯化女贞子中齐墩果酸的方法进行优化研究.以齐墩果酸的吸附率和解吸附率为考察指标,筛选出D101、D201、LSD001、XDA-7、C008、LSA-10、LSA-40、LSA-21等8种树脂中最适合齐墩果酸分离的树脂,并确定其分离纯化女贞子中齐墩果酸的最佳工艺条件.结果最优树脂为LSA-21型,最佳工艺条件为女贞子溶液浓度为4.0mg/mL,吸附流速为3mL/min,上样量2BV,洗脱剂为无水乙醇,解吸流速为3mL/min,洗脱剂用量为1.5BV.洗脱液经浓缩重结晶得结晶,经与齐墩果酸对照品进行TLC检查及熔点测定,确定其为齐墩果酸.

Study on the oleanolic acid in ligustrum Iucidum purification process

This study aimed to develop a purification method of oleanolic acid with macroreticular absorption resin.Take the adsorption rate,adsorption and desorption rate as the inspection target,filter out the most suitable for the separation of oleanolic acid resin from D101、D201、LSD001、XDA-7、C008、LSA-10、LSA-40、LSA-21 and determine the process conditions of the separation and purification oleanolic acid from Ligustrum lucidum Ait.The best is LSA-21 resin.The technological conditions are absorption concentration was about 4.0 mg/mL,flow rate was at 3 mL/min,adsorbent dosage was 2 BV,ethanol was used for eluent.The desorption speed of flow is the 3 mL/min,eluent amount used is 1.5 BV.The eluent through concentrated,ethyl alcohol dissolves,the activated charcoal decolorizes,the heavy crystallization obtains the crystalline powder.TLC check with oleanolic acid reference substance,melting point,determined it is the oleanolic acid.