| 响应面试验优化超声辅助提取连钱草多糖工艺及其体外抗氧化活性 |
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| 引用本文： | 刘晓鹏,张俊霞,姜宁,宋宜枝,向极钎,王秋霜,吴皓.响应面试验优化超声辅助提取连钱草多糖工艺及其体外抗氧化活性[J].食品科学,2016,37(4):13-19. |
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| 作者姓名： | 刘晓鹏 张俊霞 姜宁 宋宜枝 向极钎 王秋霜 吴皓 |
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| 作者单位： | 1.湖北民族学院生物科学与技术学院，湖北 恩施 445000；2.南京医药股份有限公司博士后工作站，
江苏 南京 210012；3.南京中医药大学博士后流动站，江苏 南京 210023 |
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| 基金项目： | “十二五”国家科技支撑计划项目(2011BAI04B04-5);江苏省“企业博士后集聚计划”项目(2011033);2013年湖北省战略性新兴(支柱)产业人才培养(生物工程)项目;2014年度湖北省本科高校“专业综合改革”试点项目(生物工程) |
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| 摘 要： | 通过中心复合试验优化超声辅助提取连钱草多糖的工艺，以1,1-二苯基-2-苦肼基（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基和2,2’-连氮基-双-（3-乙基苯并噻唑-6-磺酸）自由基（2,2’--azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical，ABTS＋?）清除能力、Fe2＋螯合力、铁离子还原抗氧化力（ferric reducingantioxidant power，FRAP）和N,N-二甲基-对苯二胺（N,N-dimethyl-p-phenylenediamine，DMPD）自由基清除能力为指标，研究连钱草多糖的体外抗氧化活性。结果表明，超声辅助提取连钱草多糖的最优工艺为pH 7.2、液固比32∶1（mL/g）、超声功率270 W、超声时间8 min，此条件下多糖提取率在4.95%～5.12%范围内。体外抗氧化活性结果显示，连钱草多糖DPPH自由基清除能力为（0.51±0.04）μmol Trolox/mg，ABTS＋?清除能力为（0.69±0.04）μmolTrolox/mg，Fe2＋螯合力为（0.51±0.29）μmol EDTA/mg，FRAP值为（3.45±0.03）μmol Trolox/mg，DMPD自由基清除能力为（0.17±0.01）μmol Trolox/mg。
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| 关 键 词： | 连钱草 多糖 超声辅助提取 响应面分析 抗氧化 |
Optimization by Response Surface Methodology of Ultrasound-Assisted Extraction and Antioxidant Activities of Polysaccharides from Glechoma longituba (Nakai) Kupr |
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| LIU Xiaopeng;ZHANG Junxia;JIANG Ning;SONG Yizhi;XIANG Jiqian;WANG Qiushuang;WU Hao.Optimization by Response Surface Methodology of Ultrasound-Assisted Extraction and Antioxidant Activities of Polysaccharides from Glechoma longituba (Nakai) Kupr[J].Food Science,2016,37(4):13-19. |
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| Authors: | LIU Xiaopeng;ZHANG Junxia;JIANG Ning;SONG Yizhi;XIANG Jiqian;WANG Qiushuang;WU Hao |
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| Affiliation: | 1. College of Biological Science and Technology, Hubei University for Nationalities, Enshi 445000, China;
2. Postdoctoral Research Station, Nanjing Medical Co. Ltd., Nanjing 210012, China;
3. Postdoctoral Research Station, Nanjing University of Chinese Medicine, Nanjing 210023, |
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| Abstract: | In this study, we optimized the ultrasound-assisted extraction of polysaccharides from the dried aboveground
part of Glechoma longituba (Nakai) Kupr. by response surface methodology (RSM) based on central composite design
and measured the antioxidant activities of the extracted polysaccharides using 1,1-diphenyl-2-picrylhydrazyl (DPPH)
radical scavenging, 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid radical (ABTS+·) scavenging, Fe2+ chelating
activity, ferric reducing antioxidant power (FRAP), and N,N-dimethyl-p-phenylenediamine radical scavenging methods.
The optimal extraction conditions were obtained as follows: pH, 7.2; liquid-to-solid ratio, 32:1 (mL/g); ultrasonic power,
270 W; and ultrasonic time, 8 min. Under these conditions, the yield of polysaccharides was 4.95%–5.12%. The extracted
polysaccharides exhibited powerful antioxidant activities. The DPPH radicals and ABTS+· scavenging activities, Fe2+
chelating activity, FRAP value and DMPD radical scavenging capacity were (0.51 ± 0.04) μmol Trolox equivalent
(TE)/mg, (0.69 ± 0.04) μmol TE/mg, (0.51 ± 0.29) μmol EDTA-2Na equivalent (EE)/mg, (3.45 ± 0.03) μmol TE/mg and
(0.17 ± 0.01) μmol TE/mg, respectively. |
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| Keywords: | Glechoma longituba (Nakai) Kupr polysaccharides ultrasound-assisted extraction response surface methodology antioxidant activity |
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