发酵巴戟天中多糖、寡糖与蒽醌复配的抗氧化活性研究

目的 研究发酵巴戟天中多糖、寡糖与蒽醌单独及复配后的抗氧化活性。 方法 通过DPPH、羟自由基、超氧自由基的清除率和线虫模型对三种有效组分的抗氧化活性进行测定。 结果 多糖、寡糖与蒽醌单独作用及复配均对活性氧自由基具有清除作用，复配组在中低浓度下清除DPPH自由基和超氧自由基具有协同抗氧化作用；以羟自由基为清除对象时，在高浓度只有蒽醌+寡糖和多糖+寡糖+蒽醌组才存在协同作用。此外，复配组分对线虫的寿命延长和运动行为能力增强作用效果普遍优于单独作用。结论 发酵巴戟天中多糖、寡糖与蒽醌的含量较为丰富，复配后具有良好的抗氧化效果，可为发酵巴戟天的开发提供基础数据。

Study on antioxidant activity of the compound of polysaccharide, oligosaccharide and anthraquinone in fermented Morinda officinalis How.

Objective To study the antioxidant activities of polysaccharides, oligosaccharides and anthraquinones in fermented Morinda officinalis How. alone and in combination. Methods The scavenging rates of 1, 1-diphenyl-2-Picrylhydrazyl radical (DPPH), hydroxyl radical and superoxide radical and Caenorhabditis elegans model were used to determine the antioxidant activities of the 3 kinds of active components. Results Polysaccharides, oligosaccharides and anthraquinone alone and in combination had a scavenging effect on reactive oxygen free radicals, and the combination group had a synergistic antioxidant effect on DPPH free radicals and superoxide free radicals at low and medium mass concentrations. When hydroxyl free radicals were used as scavengers, only the anthraquinone + oligosaccharide and polysaccharide + oligosaccharide + anthraquinone groups showed synergistic effects at high mass concentrations. In addition, the effects of the compound components on Caenorhabditis elegans lifespan extension and enhanced locomotor behavior were generally more effective than those of the Caenorhabditis elegans alone. Conclusion The content of polysaccharides, oligosaccharides, and anthraquinones in fermented Morinda officinalis How. are rich, have a good antioxidant effect and provide basic data for the development of fermented Morinda officinalis How.