| CPP生产副产物——CNPP酶膜耦合法制备ACE抑制肽研究 |
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| 引用本文： | 魏春燕,顾瑾,杨瑞金,赵伟,华霄,张文斌.CPP生产副产物——CNPP酶膜耦合法制备ACE抑制肽研究[J].北京轻工业学院学报,2010(6):18-22. |
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| 作者姓名： | 魏春燕 顾瑾 杨瑞金 赵伟 华霄 张文斌 |
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| 作者单位： | [1]江南大学化学与材料工程学院,江苏无锡214122 [2]江南大学食品学院,江苏无锡214122 |
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| 基金项目： | 国家高技术研究发展计划项目（2008AA10Z313） |
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| 摘 要： | 采用酶解与膜分离耦合的方法,以酶膜耦合法生产酪蛋白非磷酸肽（CPP）得到的副产物——酪蛋白非磷酸肽（CNPP）为原料制备高活性ACE抑制肽.用Protease N、胰蛋白酶、Fla-vourzyme、AS1398、酸性蛋白酶等几种蛋白酶对经过酶膜耦合制备的酪蛋白非磷酸肽进行二次酶解,同时与1 K膜分离耦合,然后对二次酶膜耦合所得产物的ACE抑制活性进行比较.结果表明,Protease N比其他几种蛋白酶更适合用于酪蛋白非磷酸肽制备高活性的ACE抑制肽,其产物的蛋白质量浓度为1 g/L时的ACE抑制率达到55.60%.
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| 关 键 词： | 酪蛋白非磷酸肽（CNPP） 酶膜耦合 ACE抑制活性 |
PREPARATION OF ACE INHIBITORY PEPTIDES FROM CASEIN NON-PHOSPHOPEPTIDES BY COUPLING ENZYMATIC HYDROLYSIS WITH MEMBRANE SEPARATION |
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| WEI Chun-yan,GU Jin,YANG Rui-jin,ZHAO Wei,HUA Xiao,ZHANG Wen-bin.PREPARATION OF ACE INHIBITORY PEPTIDES FROM CASEIN NON-PHOSPHOPEPTIDES BY COUPLING ENZYMATIC HYDROLYSIS WITH MEMBRANE SEPARATION[J].Journal of Beijing Institute of Light Industry,2010(6):18-22. |
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| Authors: | WEI Chun-yan GU Jin YANG Rui-jin ZHAO Wei HUA Xiao ZHANG Wen-bin |
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| Affiliation: | 1.School of Chemical and Material Engineering,Jiangnan University,Wuxi 214122,China;2.School of Food Science and Technology,Jiangnan University,Wuxi 214122,China) |
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| Abstract: | Peptides with high ACE-inhibitory activity were prepared from Casein non-phosphopeptides（CNPP） which was the by-products of Casein phosphopeptides（CPP） by coupling enzymatic hydrolysis with membrane separation.Five enzymes（Protease N,trypsin,Flavourzyme,AS1398,Acid protease） were employed to hydrolyze CNPP for the second time and meanwhile 1K membrane was chosen to separate the product.Results showed that Protease N has an obvious superiority other other enzymes in preparation of high ACE-inhibitory activity peptides.The ACE-inhibitory activity was 56.08% when the protein concentration was 1 g/L. |
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| Keywords: | casein non-phosphopeptides（CNPP） coupling of enzymatic hydrolysis and membrane separation ACE-inhibitory activity |
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