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Impact of Initial pH and Linoleic Acid (C18:2) on Hydrogen Production by a Mesophilic Anaerobic Mixed Culture |
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| Authors: | Srimanta Ray Nabin Chowdhury Jerald A. Lalman Rajesh Seth Nihar Biswas |
| Affiliation: | 1Graduate Student, Dept. of Civil and Environmental Engineering, Univ. of Windsor, 335 Essex Hall, 401 Sunset Ave., Windsor, Ontario, Canada N9B 3P4. E-mail: rays@webmail1.uwindsor.ca 2Graduate Student, Dept. of Civil and Environmental Engineering, Univ. of Western Ontario, London, Ontario, Canada N6A 5B9. E-mail: nchowdh3@uwo.ca 3Associate Professor, Dept. of Civil and Environmental Engineering, Univ. of Windsor, 334 Essex Hall, 401 Sunset Ave., Windsor, Ontario, Canada N9B 3P4 (corresponding author). E-mail: lalman@uwindsor.ca 4Assistant Professor, Dept. of Civil and Environmental Engineering, Univ. of Windsor, B07 Essex Hall, 401 Sunset Ave., Windsor, Ontario, Canada N9B 3P4. E-mail: rseth@uwindsor.ca 5Professor, Dept. of Civil and Environmental Engineering, Univ. of Windsor, 147 Essex Hall, 401 Sunset Ave., Windsor, Ontario, Canada N9B 3P4. E-mail: biswas@uwindsor.ca |
| Abstract: | Hydrogen production from glucose using a mixed anaerobic culture was assessed under batch conditions by adjusting the initial pH and adding linoleic acid (LA). At an initial pH of 5.0, hydrogen (1.9?mol?H2?mol?1 glucose) was detected after the first glucose injection in the controls (no LA added). At the latter initial pH and in cultures fed 2,000?mg?l?1 LA, the yield reached a maximum of 2.4?mol?H2?mol?1 glucose when glucose was injected again. Hydrogen was detected after the second glucose injection in cultures with the initial pH adjusted to 5.0 and 6.0. In cultures receiving LA and adjusted to pH 7.6, the hydrogen yield reached 1.4?mol?H2?mol?1 glucose when glucose was injected again. Acetate, propionate, and butyrate were detected under all conditions; however, the quantities were variable and dependent on the conditions examined. In comparison to the amount of volatile fatty acids produced, relatively low quantities of alcohols (ethanol, i-propanol, n-butanol, and i-butanol) were detected during the initial lag phase of 96?to?120?h. |
| Keywords: | Anaerobic treatment Hydrogen Gas pH Acids Waste management |