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Sporulation boundaries and spore formation kinetics of Bacillus spp. as a function of temperature, pH and a(w)
Authors:Eugénie Baril  Louis Coroller  Olivier Couvert  Mohammed El Jabri  Ivan Leguerinel  Florence Postollec  Christophe Boulais  Frédéric Carlin  Pierre Mafart
Affiliation:Université Européenne de Bretagne, France; Université de Brest, EA3882, Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, UMT 08.3 PHYSI'Opt, IFR148 ScInBioS, 6 rue de l'Université, F-29334 Quimper, France; ADRIA Développement, UMT 08.3 PHYSI'Opt, Z.A. Creac'h Gwen, F-29196 Quimper Cedex, France.
Abstract:Sporulation niches in the food chain are considered as a source of hazard and are not clearly identified. Determining the sporulation environmental boundaries could contribute to identify potential sporulation niches. Spore formation was determined in a Sporulation Mineral Buffer. The effect of incubation temperature, pH and water activity on time to one spore per mL, maximum sporulation rate and final spore concentration was investigated for a Bacillus weihenstephanensis and a Bacillus licheniformis strain.
Keywords:Bacillus weihenstephanensis  Bacillus licheniformis  Sporulation boundaries  Growth  Temperature  pH  Water activity  Modeling
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