On-column solvent exchange for purified preparative fractions |
| |
Authors: | Goetz Gilles H Beck Emily Tidswell Peter W |
| |
Affiliation: | 1Pfizer Global Research and Development, Analytical Chemistry and Sample Logistics, St Louis Laboratories, Chesterfield, MO;2School of Medicine, Washington University, St Louis, MO |
| |
Abstract: | On-column solvent exchange, using many of the principles of solid-phase extraction, has been implemented to significantly reduce evaporation cycle time following reverse-phase preparative HPLC. Additional benefits, such as a reduced potential for salt formation, thermal decomposition, and residual solvent, are also described. Fractions obtained from preparative separations, typically in a large volume of acetonitrile:water, are injected into the preparative HPLC and then eluted in acetonitrile, creating a new fraction in a volatile organic solvent. Minimal modification to the instrument was required, and unattended operation is possible. Acetonitrile evaporation is achieved within 3 h, compared with 17 h for aqueous-based fractions; lower temperatures can be used during the evaporation step; mobile-phase additives, likely to form salts with the target compound if concentrated in the fraction, are removed before evaporation; sample recovery and purity are unaffected. |
| |
Keywords: | fraction concentration solvent exchange solid-phase extraction preparative chromatography |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|