灵芝菌丝体深层发酵工业化生产的研究

目的:采用生物发酵工程技术进行灵芝菌丝体多糖扩大生产;方法:利用农副产品(如玉米粉、豆粕粉)作培养基,在发酵罐中,根据其控制参数(如接种量、总糖量、还原糖、pH值、通风量、罐压、温度、生长因子),进行灵芝菌丝体多糖工业化扩大生产。结果:经过20批容积为10m3,定容为7.5m3发酵罐正常试验,生产周期平均为150h;pH值从6.5降至3.5;放罐后灵芝菌丝体经均质和喷雾干燥后其干粉平均每罐重量为66.1kg,干粉平均收率为8.76kg/m3,干粉中纯多糖含量为68.5g/kg,每罐纯多糖重量为4.225kg。镜检菌丝壁上无明显“芽头与锁状联合”,并有少量菌丝体自溶,无杂菌。结论:试验证明本工艺方案是成功的,是值得推广的新工艺。

Study on Commercial Production from Deep Submerged Fermentation of Ganoderma lucidum Mycelia

Objective: By bio-fermentation engineering to expand production of Ganoderma lucidum polysaccharide. Method: Minor farm products (such as maize powders, soybean powders) were used as culture media, after controlling parameters in fermenter (such as inoculation quantity, total saccharide quantity, pressure of fermenter, temperature and growth factor etc.) to increase production. Result: Twenty normal tests were studied. The volume of every fermenter was 10m3, the fermentation liquid 7.5m3, the average production cycle 150h and pH reduced from 6.5 to 3.5. After the mycelia fermented, the average weight of dry powders in every fermenter is 66.1kg by spray drying, the average recovery 8.76kg/m3, the content of pure polysaccharide 68.5g/kg in the dry powders and the weight of the pure polysaccharide 4.225kg in every fermenter. There was no obvious vgerminate and lock like connection on the mycelia wall. There was a little mycelia dissolved with no other bacteria. Conclusion: It proves that the technology is successful. It is valuable to popularize.