RRLC-Q-TOF MS/MS法分析生晒参和大力参中的皂苷类成分

利用高分离度快速液相色谱与四极杆-飞行时间质谱（RRLC-Q-TOF MS/MS）联用，对生晒参和大力参中的人参皂苷类成分进行比较研究。基于人参皂苷Rd和Re、Rf和Rg₁两组同分异构体在串联质谱中的裂解规律，以及mRd的串联质谱特征，通过标准品保留时间和精确分子质量鉴定了人参中的35种人参皂苷，进而比较了生晒参和大力参中人参皂苷的相对百分含量差异，并于大力参中检测到稀有人参皂苷，如人参皂苷Rg₅、Rk₁、F₄、Rg₆ 等。该方法对于大力参类传统中药炮制品的成分研究具有借鉴意义，研究结果能说明大力参区别于生晒参的物质基础，可以为大力参在临床上合理、有效地使用提供指导。

Study on Ginsenosides in White Ginseng and Dali Ginseng by RRLC-Q-TOF MS/MS

Rapid resolution liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry (RRLC-Q-TOF MS/MS) was used to compare the white ginseng with dali ginseng in the composition of ginsenosides. Based on the fragmentation law of the isomeric compounds, ginsenoside Rd and Re, ginsenoside Rf and Rg₁, and the feature of ginsenoside mRd in MS/MS, 35 kinds of ginsenosides were identified by the comparison of the retention time of the standard compound and the accurate mass obtained from RRLC-Q-TOF MS/MS. The rare ginsenosides were detected by the comparison of white ginseng with dali ginseng, such as ginsenoside Rg₅， Rk₁, F₄， Rg₆ etc. The methods has reference significance for the research of compounds in the processed products of herbs, like dali ginseng. The results provide the reference for clinical rational drug use.