Hydroxypropyl β-cyclodextrin improving multiple stresses tolerance of Lactococcus lactis subsp. lactis |
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Authors: | Lianming Cui Songyang Lin Juanjuan Yi Xin Liu Limin Hao Yizhi Ji Laizheng Lu Zhenyu Ji Qiaozhen Kang Jike Lu |
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Affiliation: | 1. School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, 450001 China;2. The Quartermaster Equipment Institute, Academy of Military Sciences PLA China, Beijing, 100010 China;3. Beijing Key Laboratory of Biomass Waste Resource Utilization, College of Biochemistry and Engineering, Beijing Union University, Beijing, 100023 China;4. Zhengzhou Mindtek Biotechnology Co. Ltd, Zhengzhou, Henan, 450001 China;5. Henan Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, Henan, 450001 China |
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Abstract: | L. lactis is known as industrial starter in the fermentation of dairy and meat products, and it plays an important role in human health as an edible probiotic. During industrial production, L. lactis often experiences different stresses that delay the growth and decrease the survival in some serious conditions. In this study, the protective effects of hydroxypropyl β-cyclodextrin (HP β-CD) on L. lactis under multiple stresses were investigated. The microbial cells were treated with different stresses including heat, NaCl, cold, and H2O2 stresses, and the results were showed by measuring the OD600 or spot plating method. The growth and tolerance were improved when HP β-CD was added during different stress conditions, better than that of trehalose. Besides, the scanning electron microscopic and fluorescence spectrum studies showed that HP β-CD could combine with L. lactis to protect the cell structure, suggesting that HP β-CD may act as a protective agent of L. lactis. Therefore, HP β-CD could be considered as a potential protective agent to be applied in food industry, and its protective mechanism on L. lactis still needs further investigation. |
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Keywords: | hydroxypropyl β-cyclodextrin Lactococcus lactis subsp Lactis protective agent stress response |
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