| 破伤风毒素的提纯及形成离子通道的特性 |
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| 引用本文： | 雷殿良,松田守弘.破伤风毒素的提纯及形成离子通道的特性[J].粉末涂料与涂装,1990(4). |
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| 作者姓名： | 雷殿良 松田守弘 |
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| 作者单位： | 中国药品生物制品检定所 北京100050(雷殿良)，大阪大学微生物病研究所 日本(松田守弘) |
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| 摘 要： | 应用硫酸铵分段沉淀和 Ultrogei AcA_(34)凝胶过滤对破伤风毒进行了提纯,并对形成的离子通道在中毒作用中的功能进行了讨论。提纯的毒素与抗粗制毒素血清形成一条沉淀线,于聚丙烯酰胺凝胶电泳中形成单一的蛋白带,分子量为15万,等电点为5.95,毒力为1～3×10~4MLD/μg 蛋白。给小鼠注射低剂量毒素引起迟发性强直性痉挛;注射高剂量毒素引起快速的类肉毒毒素的松弛性麻痹,直至死亡。应用 Patch clamp 技术,破伤风毒素于人工磷脂膜上形成离子通道。只有当膜两侧存有 pH 梯度时毒素才形成离子通道,因此是 pH 依赖的。通道的电导系数为9 PS。
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| 关 键 词： | 破伤风毒素 毒力 离子通道 |
Purification of Tetanus Toxin and Formation of Ion Channels in a Lipid Bilayer |
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| Lei Dianliang National Institute for the Control of Pharmaceutical and Biological Products,Beijing
Matsuda M Research Institute for Microbial Diseases,Osaka University,Osaka.Purification of Tetanus Toxin and Formation of Ion Channels in a Lipid Bilayer[J].Chinese Journal of Biologicals,1990(4). |
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| Authors: | Lei Dianliang National Institute for the Control of Pharmaceutical and Biological Products Beijing
Matsuda M Research Institute for Microbial Diseases Osaka University Osaka |
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| Abstract: | Abstract Tetanus toxin was purified by ammonium sulphate fractionation and gel filtration on a column of Ultrogel AcA_(34) from culture filtrate of Clostridium teta- ni.Purified toxin with a toxicity of 1～3×10~4 MLD/μg Protein,formed single pr- ecipitation line by immunodifusion against horse anti-crude toxin.It migrated as a single protein band in PAGE and SDS-PAGE with a molecular weight of 150000 Da- lton.The pI was 5.95.It elicited delayed spastic toxicity in mice in low doses of toxin and acute botulism-like flaccid toxicity in high doses.The toxin created ionch- annels in a lipid bilayer by a Patch Clamp technique,with a conductance of 9 pS. The channel forming activity of tetanus toxin was pH dependent.The function of channel forming activity in the intoxication of tetanus toxin was discussed. |
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| Keywords: | Tetanus toxin Toxicity Ion channel |
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