| 鱼藤酮的高效液相色谱分析方法研究 |
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| 引用本文： | 武永昆,林军,叶敏. 鱼藤酮的高效液相色谱分析方法研究[J]. 云南化工, 2006, 33(2): 59-60 |
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| 作者姓名： | 武永昆 林军 叶敏 |
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| 作者单位： | 1. 云南农业大学,农药系,云南,昆明,650201;云南中科生物产业有限公司,云南,昆明,650106
2. 云南大学,应用化学系,云南,昆明,650091 |
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| 摘 要： | 介绍一种鱼藤酮的高效液相色谱定量分析方法。采用hypersil C18色谱柱,在以v(乙腈)∶v(水)=60∶40为流动相,流速为1.0 mL/m in,检测波长为294 nm的条件下,用外标法对鱼藤酮原药中的有效成分进行定量分析。方法的标准差为0.28,变异系数为0.39%,线性相关系数为0.9999,平均回收率为99.54%。
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| 关 键 词： | 鱼藤酮 高效液相色谱 定量分析 |
| 文章编号： | 1004-275X（2006）02-0059-02 |
| 收稿时间： | 2005-11-09 |
| 修稿时间： | 2005-11-09 |
Determination of Rotenone by High Performance Liquid Chromatography |
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| WU Yong-kun,LIN Jun,YE Min. Determination of Rotenone by High Performance Liquid Chromatography[J]. Yunnan Chemical Technology, 2006, 33(2): 59-60 |
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| Authors: | WU Yong-kun LIN Jun YE Min |
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| Affiliation: | 1. Department of Pesticide Science, Yunnan Agricuhural University, Kunming 650201, China; 2. Yunnan Zhongke Bio- Industry Co. , Limited, Kunming 650106, China; 3. Department of Applied Chemistry, Yunnan University, Kunming 650091, China |
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| Abstract: | A HPLC method for the determination of rotenone was introduced.The test sample were separated on a hypersil C18 column eluting with acetonitrile/water(60/40,V/V) at 1.0 ml/min flow ratio and detected at 294 nm.Results showed that standard deviation,variation coefficient and average recovery was 0.28,0.39% and 99.54% respectively. |
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| Keywords: | rotenone HPLC quantitative analysis |
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