导数分光光度法同时测定烃水合产物中仲,叔醇的含量

考查了C_4—C_6仲醇和叔醇的亚硝酸酯导数光谱行为.确定用二阶导数以峰零法在366.4nm波长下测定叔醇,在368.2nm 波长下测定仲醇。仲醇的吸光系数 k_(?)=2.18 l/g·cm,叔醇的k_(366.4nm)=3.49 l/g·cm。分别约为常规分光光度法峰值吸收系数的2倍及4倍。样品分析结果表明,仲醇回收率在95—101%,叔醇回收率在97—106%,变异系数<3.5%,相对误差<±3%,仲醇最低检出限为 3μg/ml,叔醇最低检出限为 1μg/ml。

Simultaneous Determination of Secondary and Tertiary Alcohols in Hydration Products of Hydrocarbon by Derivative Absorption Spectrophotometry

Derivative absorption spectra of nitrous ester of secondary and tertiary alcoholswere studied.Secondary alcohols were determined at 368.2nm and tertiary alcoholat 366.4nm by the secondary derivative absorption spectrophotometry.Absorptivi-ties of secondary and tertiary alcohols are 2.18 and 3.49 1/g·cm and being twoand four times of that of conventional spectrophotometry.Sample analysis resultsindicate recovery of secondary alcohol being 95—101%,that of tertiary alcohol,97—106%.Coefficient of variation is less than 3.5,relative error is less than±3%.The detection limit is 3μg/ml for secondary alcohol and 1μg/ml for terti-ary alcohol.