负压双管线流动注射离子选择电极法同时测定血清K+、Na+、Cl-、Ca2+

为了简化装置结构、降低仪器成本、提高分析系统的稳定性和抗干扰能力,推出一种全新的负压式双管线流动注射离子选择电极分析方法,并对该方法相关的载液组成及流量、载流酸度调节液的组成及流量,反应盘管长度等进行了优化,最后将其成功用于血清K+、Na+、Cl-、Ca2+的同时测定。方法的相对标准偏差<1.3%(n=11),回收率为96%～104%,分析速度为480检出/h,样品耗量仅为45μL/次;对血清K+、Na+、Cl-、Ca2+的同时测定范围分别是2.0～24.0、102～252、20～300、0.70～8.0 mmol.L-1,此测定范围涵盖了正常人血清中的电解质浓度。

A negative-pressure double-lines flow-injection ion-selective electrode method for simultaneous determination of K+/Na+/Cl-/Ca+ in serums

In order to simplify the device configuration,reduce the cost,improve the instrument stability and anti-interference ability,a new type of double-line flow-injection ion-selective electrode method was proposed,and determination conditions of the method,such as the carrier composition and flow rate,acidity adjusting liquid composition and flow rate,and reaction coil length were optimized.The method was successfully used in simultaneous determinations of K+、Na+、Cl-、Ca2+ in serums.Linear ranges of the method were in 2.0~24.0 mmol·L-1 for K+,102~252 mmol·L-1 for Na+,20~300 mmol·L-1 for Cl-and 0.7~8.0 mmol·L-1 for Ca2+,respectively.The relative standard deviation was less than 1.3%(n=11),the recoveries obtained by adding K+,Na+,Cl-and Ca2+ in serum samples were in range 96%~104%,the analysis speed was 480 detections/h,and the consumption of the sample was only 45 μL per time.These determination ranges were in normal ranges of human serum’s electrolyte concentrations.