| 沙门氏菌的依赖于核酸序列恒温扩增检测方法的建立 |
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| 引用本文： | 雷质文,姜英辉,王妍婷,赵丽青,张健,倪鑫,王建广,梁成珠. 沙门氏菌的依赖于核酸序列恒温扩增检测方法的建立[J]. 食品安全质量检测学报, 2011, 2(5): 248-252 |
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| 作者姓名： | 雷质文 姜英辉 王妍婷 赵丽青 张健 倪鑫 王建广 梁成珠 |
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| 作者单位： | 山东出入境检验检疫局技术中心;;山东出入境检验检疫局技术中心;;山东出入境检验检疫局技术中心;;山东出入境检验检疫局技术中心;;山东出入境检验检疫局技术中心;;中国海洋大学;山东出入境检验检疫局技术中心;;山东出入境检验检疫局技术中心; |
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| 摘 要： | 采用自行建立和优化的依赖于核酸序列恒温扩增(nucleic acid sequence-based amplification,NASBA)检测体系,对沙门氏菌进行检测。采用沙门氏菌的invA基因为目的片段设计特异性引物,建成可快速检测沙门氏菌的NAS-BA检测法,并进行了特异性和灵敏度试验。结果表明:所建立起的NASBA检测方法,灵敏度为7.1×102cfu/ml,高于普通PCR方法。
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| 关 键 词： | 沙门氏菌 检测 依赖于核酸序列恒温扩增 |
Establishment of the nucleic acid sequence-based amplification method of detecting Salmonella |
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| Lei Zhiwen,Jiang Yinghui,Wang Yanting,Zhao Liqing,Zhang Jian,Ni Xin,Wang Jianguang and Liang Chengzhu. Establishment of the nucleic acid sequence-based amplification method of detecting Salmonella[J]. Journal of Food Safety & Quality, 2011, 2(5): 248-252 |
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| Authors: | Lei Zhiwen Jiang Yinghui Wang Yanting Zhao Liqing Zhang Jian Ni Xin Wang Jianguang Liang Chengzhu |
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| Affiliation: | Technical ceter for Shandong Entry-Exit Inspection and Quarantine Bureau; Qingdao 266002; China;;Technical ceter for Shandong Entry-Exit Inspection and Quarantine Bureau; Qingdao 266002; China;;Technical ceter for Shandong Entry-Exit Inspection and Quarantine Bureau; Qingdao 266003; China;;Technical ceter for Shandong Entry-Exit Inspection and Quarantine Bureau; Qingdao 266004; China;;Technical ceter for Shandong Entry-Exit Inspection and Quarantine Bureau; Qingdao 266005; China;;Institute of Food Science and Engineering; Ocean University of China; Qingdao266003; China;Technical ceter for Shandong Entry-Exit Inspection and Quarantine Bureau; Qingdao 266005; China;;Technical ceter for Shandong Entry-Exit Inspection and Quarantine Bureau; Qingdao 266005; China; |
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| Abstract: | A new method,which based on Nucleic Acid Sequence-based Amplification(NASBA) to detect Salmonella of sample,was established.A specific set of primers was synthesized to target the invA gene of Salmonella so as to establish Nucleic Acid Sequence-based Amplification method.Specific and sensitivity were tested.The results shows that the sensitivity of NASBA was 7.1×102 cfu/ml which was higher than the result of PCR method.Detecting Salmonella with NASBA was more specific and sensitive than PCR method and has l... |
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| Keywords: | salmonella detection nucleic acid sequence-based amplification |
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