Evaluation of hidden H2-consuming pathways using metabolic flux-based analysis for a fermentative side-stream dynamic membrane bioreactor using untreated seed sludge |
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Authors: | Mubbshir Saleem Razieh Rafieenia Jalal Uddin Maria Cristina Lavagnolo |
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Affiliation: | 1. University of Padova, Department of Chemical Sciences, University of Padova, Via Marzolo 1, 35131, Padova, Italy;2. Department of Microbial Sciences, University of Surrey, Guildford, GU27XH, United Kingdom;3. Department of Pharmaceutical Chemistry, College of Pharmacy, King Khalid University, Abha 61441, Saudi Arabia;4. Department of Civil, Environmental and Architectural Engineering, University of Padova, Via Marzolo 9 35131 Padova, Italy |
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Abstract: | This study investigates the performance and hidden hydrogen consuming metabolic pathways of a fermentative side stream dynamic membrane (DM) bioreactor using flux balance analysis (FBA). The bioreactor was inoculated with untreated methanogenic seed sludge. It was found that fouling rate aggravated with increasing COD concentration (10–30 g/L) and was positively correlated to it rather than to the applied solid flux on the DM module. Due to increased fouling rate the hydraulic retention time (HRT) could not be reduced less than 0.82 ± 0.02 d. An increase in the organic loading rate (OLR) led to an increase in H2 yield from 0.01 to 0.76 mol H2/mol of sucrose. FBA revealed that homoacetogenesis was the main H2-consuming pathway at lower OLRs (corresponding to 10 and 15 g COD/L), while for the OLR corresponding to 30 g COD/L, homoacetogens were suppressed. More importantly, caproic acid production pathway was identified for the first time as another H2-consuming pathway at high OLR which was not significant at lower OLRs during fermentative dynamic membrane bioreactor operations. |
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Keywords: | Dynamic membranes Dark fermentation Flux balance analysis (FBA) Fouling Biological hydrogen production |
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