牡蛎功能短肽的制备及ACE抑制活性

以新鲜无壳牡蛎为原料,采用酶水解的方法制备牡蛎短肽,经SephadexG 15分离,并用HPLC测定其相对分子质量分布,通过HPLC法定量马尿酸测定各组分的ACE(血管紧张素转化酶)抑制活性。结果表明,牡蛎水解液中相对分子质量较大和较小部分的ACE抑制活性偏低,只有相对分子质量在一定范围内的短肽,对ACE具有较好的抑制作用,质量浓度为0.4mg/mL的牡蛎功能短肽的ACE抑制率为51.4%.

Preparation of Oyster Functional Oligopeptides and Its ACE Activity Inhibition Capability

To prepare Oyster Functional Oligopeptides (OFO) with ACE activity inhibition capability, The fresh oysters without shells was hydrolysed by a microbial protease alcalase. The OFO were separated from the hydrolyzate by Sephadex G-15, their molecular weight distribution was determined by HPLC, and the ACE activity inhibitory capability grouped with different molecular weights was assayed by HPLC method. The results showed that OFO with high and low molecular weights had low ability in inhibiting ACE activity, and only short chain peptides with a certain molecular weight range had high ability. The inhibition rate was up to 51.4% at the concentration of 0.4 mg/mL.