| 等度高效液相色谱测定大豆异黄酮中大豆甙元和金雀异黄酮 |
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| 引用本文： | 林疆,杨兰钦,唐根源. 等度高效液相色谱测定大豆异黄酮中大豆甙元和金雀异黄酮[J]. 福建分析测试, 2006, 15(2): 15-16,20 |
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| 作者姓名： | 林疆 杨兰钦 唐根源 |
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| 作者单位： | 福州福龙生物制品有限公司,福建,福州,350015;福州福龙生物制品有限公司,福建,福州,350015;福州福龙生物制品有限公司,福建,福州,350015 |
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| 摘 要： | 本文介绍了等度高效液相色谱(HPLC)测定大豆异黄酮中二种主要成份:大豆甙元(Daidzein)和金雀异黄酮(Genistein)的方法。采用Atlantis C18色谱柱和EasyGuard C18,保护柱;以甲醇:0·1%醋酸(pH3·11)=51·5:48·5(v/v)为流动相;检测波长λ=254nm;流速1mL/min。在测定范围内(10-200ng)峰面积与质量浓度线性关系良好。大豆甙元和金雀异黄酮的相关系数分别为0·9984,0·9997·两组份回收率为97·0—102·92%·
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| 关 键 词： | 高效液相色谱 大豆异黄酮 大豆甙元 金雀异黄酮 |
| 文章编号： | 1009-8143（2006）02-0015-02 |
| 收稿时间： | 2005-07-02 |
| 修稿时间： | 2005-07-28 |
Determination of the Daidzein and the Genistein in the Soy Bean Isoflanoves by Isocratic Elution High Performance Liquid Chromatography |
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| Lin Jiang,Yang Lanqin,Tang Genyuan. Determination of the Daidzein and the Genistein in the Soy Bean Isoflanoves by Isocratic Elution High Performance Liquid Chromatography[J]. Fujian Anal Ysis & Testing, 2006, 15(2): 15-16,20 |
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| Authors: | Lin Jiang Yang Lanqin Tang Genyuan |
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| Abstract: | An analytical approach has been developed for isocratic elution high performance liquid chromatographic determination of Daidzein and Genistein in the soy bean isoflanoves.They were measured using Atlantis C_ 18 for analytical column and EasyGuard column C_ 18 for guard column,and UV detector(at 254nm).The mobile phase was methanol : 0.1% acetic acid(pH3.11) = 51.5:48.5(v/v).there was a good linear relationship between the content of Daidzein,Genistein and its peak area,with the correlation coefficients of 0.9984 and 0.9997 respectively,Average recoveries were 97.0%-103.0%. |
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| Keywords: | High performance liquid chromatography Soy bean isoflanoves Daidzein Genistein |
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