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Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase |
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| Authors: | MM Gvozdenović BZ Jugović DI Bezbradica MG Antov ZD Knežević-Jugović BN Grgur |
| Affiliation: | 1. Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia;2. Institute of Technical Science, Serbian Academy of Science and Arts, Knez Mihajlova 35, 11000 Belgrade, Serbia;3. Faculty of Technology, University of Novi Sad, Blvd. Cara Lazara 1. 21000 Novi Sad, Serbia |
| Abstract: | Polyaniline (PANI) enzyme electrode was formed by immobilisation of Glucose oxidase (GOx) via glutaraldehyde into electrochemically polymerised PANI on graphite electrode. Electrochemical polymerisation of PANI on graphite was performed from aqueous solution of 1.0 mol dm−3 HCl and 0.25 mol dm−3 aniline at constant current density of 2.0 mA cm−2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm−2 were recorded in different glucose concentrations. The linearity response range was between 1.0 and 5.0 mmol dm−3 of glucose concentration. The estimated apparent Michaelis–Menten constant, was Km′ = 0.30 mmol dm−3, which is significantly lower than that of free enzyme. |
| Keywords: | Glucose oxidase Immobilisation Electrochemical synthesis Polyaniline Biosensors |
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