|
|||||
|
|
In vivo antitumoural activity and composition of an oil extract of Brazilian propolis |
|
| Authors: | Adriana Andrade Carvalho Daiane Finger Christiane Schinieder Machado Eduardo Morgado Schmidt Patrícia Marçal da Costa Ana Paula Negreiros Nunes Alves Thamires Maria Fontenele Morais Maria Goretti Rodrigues de Queiroz Sueli Pércio Quináia Marcos Roberto da Rosa Julio Murilo Trevas dos Santos Cláudia Pessoa Manoel Odorico de Moraes Letícia Veras Costa-Lotufo Alexandra Christine Helena Frankland Sawaya Marcos Nogueira Eberlin Yohandra Reyes Torres |
| Affiliation: | 1. Departamento de Fisiologia, Universidade Federal do Ceará, Fortaleza, CE, Brazil;2. Departamento de Química, Universidade Estadual do Centro-Oeste, Guarapuava, PR, Brazil;3. Departamento de Clínica Odontológica, Universidade Federal do Ceará, Fortaleza, CE, Brazil;4. Faculdade de Farmácia, Universidade Federal do Ceará, Fortaleza, CE, Brazil;5. Departamento de Fisiologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brazil;6. Laboratório ThoMSon de Espectrometria de Massas, Instituto de Química, Universidade Estadual de Campinas, Campinas, SP, Brazil |
| Abstract: | The present study aimed to evaluate in vivo and in vitro the antitumoural activity of a propolis extract obtained with edible vegetable oil and its fractions and also to investigate its chemical composition by LC–MS and LC–MS/MS. To evaluate the toxicological aspects related to the propolis extract treatment, hematological, biochemical, histopathological and morphological analyses of treated animals were performed. All propolis extracts showed an in vivo antitumour activity in the experimental model with a moderate toxicity effect at experimental exposure levels. The oil extract was as effective as the ethanolic extract at inhibiting tumour growth. In vitro assays showed that the whole oil extract produced better inhibition of tumour cells than its fractions. LC–MS and LC–MS/MS identified four phenolic acids and three flavonoids. The anticancer potential of the oil extract of propolis has been demonstrated and the edible vegetable oil was shown as an attractive alternative solvent to extract bioactive natural propolis components. |
| Keywords: | Propolis Edible oil extract Antitumour activity Phenolic compounds |
| 本文献已被 ScienceDirect 等数据库收录！ | |