Protein expression and enzymatic activities in normal and soft textured Atlantic salmon (Salmo salar) muscle |
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Authors: | Iciar Martinez,På l Anders Wang,Rasa Slizyté ,Alberto Jorge,Stine W. Dahle,Benito Cañ as,Michiaki Yamashita,Ragnar L. Olsen,Ulf Erikson |
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Affiliation: | 1. Marine Research Institute, CSIC, Eduardo Cabello 6, E-36208 Vigo, Spain;2. The Norwegian College of Fishery Science, University of Tromsø, N-9037 Tromsø, Norway;3. SINTEF Fisheries and Aquaculture, Brattørkaia 17C, N-7465 Trondheim, Norway;4. Museo de Ciencias Naturales, CSIC, Madrid, Spain;5. Dept. of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense de Madrid, Madrid, Spain;6. National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanazawa-ku, Yokohama 236-8648, Japan |
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Abstract: | Soft textured Atlantic salmon is a sporadic and occasionally very severe problem for the farming and processing industries. The firm and soft fillets examined in this work differed in their gelatinase activities, cross-reactivity with anti-ubiquitin and anti-cathepsin L antibodies, as well as in the in-gel α-chymotryptic peptide maps of electrophoretically isolated myosin heavy chain (MHC) bands. The immunodetections of actin, α-actinin, MHC, and the MALDI TOF MS peptide mass fingerprinting of electrophoretically isolated MHCs only showed minor differences between samples. Other analyses revealed merely individual differences. These results seem to indicate a higher level of gelatinase activation, ubiquitination and cathepsin L cross-reacting material in softer muscle. These results would be consistent with a myopathy, but also with what could be expected in the skeletal muscle of healthy salmonid fish during a normal period of hyperplastic growth. |
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Keywords: | Fish muscle Fish texture Soft muscle Salmo salar Farmed fish Muscle proteases MMPs Cathepsin L Ubiquitination |
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