Identification and quantitative determination of glucosinolates in seeds and edible parts of Korean Chinese cabbage |
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Authors: | Eunyoung Hong Sun-Ju Kim Gun-Hee Kim |
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Affiliation: | 1. Department of Food and Nutrition, Duksung Women’s University, Seoul 132-714, Republic of Korea;2. Department of Biological Environment and Chemistry, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Republic of Korea |
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Abstract: | Glucosinolates (GSLs) have attracted major interest due to the chemopreventive properties of some of their transformation products. GSLs in the seeds and edible parts of Korean Chinese cabbage (Brassicacampestris L. ssp. pekinensis) were identified and quantified by LC–ESI–MS and LC–UV. As a result, nine GSLs were identified: progoitrin, glucoraphanin, glucoalyssin, gluconapin, 4-hydroxy-3-indolylmethyl, glucobrassicanapin, glucoerucin, glucobrassicin, and 4-methoxyglucobrassicin. The total GSL levels were 268–198 and 23.0–15.8 μmol/g dry weight (DW) for seeds and edible parts, respectively. Gluconapin (197 μmol/g DW) was the highest individual GSL in seeds, whereas 4-methoxyglucobrassicin (6.08–4.94 μmol/g DW) and glucobrassicanapin (8.18–3.09 μmol/g DW) were found in the edible parts. In addition, LC–MS profiles of the nine GSLs identified from Korean Chinese cabbage were subjected to principal components analysis (PCA) to evaluate differences among samples. The metabolome among the four cultivar seed or edible parts was clearly separated by PCA. |
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Keywords: | Korean Chinese cabbage Glucosinolates LC&ndash ESI&ndash MS Metabolic profiles |
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