Tissue turnover in ovine muscles and lipids as recorded by multiple (H,C, O,S) stable isotope ratios |
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Authors: | Sabine M. Harrison Olaf Schmidt Aidan P. Moloney Simon D. Kelly Andreas Rossmann Antje Schellenberg Federica Camin Matteo Perini Jurian Hoogewerff Frank J. Monahan |
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Affiliation: | 1. UCD School of Agriculture, Food Science & Veterinary Medicine, Agriculture and Food Science Centre, Belfield, Dublin 4, Ireland;2. Teagasc, Grange Beef Research Centre, Dunsany, Co. Meath, Ireland;3. Food and Environment Research Agency, Sand Hutton, York YO41 1LZ, United Kingdom;4. Isolab GmbH, 85301 Schweitenkirchen, Germany;5. LGL Bayern, Dienststelle Oberschleißheim, 85764 Erlangen, Germany;6. IASMA–Edmund Mach Foundation, Via Mach 1, San Michele all’Adige, Trento, Italy;g UEA School of Chemical Sciences and Pharmacy, Norwich NR4 7TJ, United Kingdom |
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Abstract: | Multiple stable isotope ratios (δ2H, δ13C, δ18O and δ34S) were measured in muscle, muscle lipids and lipid fractions collected from 28 lambs, subjected to a diet-switch and raised on two energy allowances (EAs), to determine tissue turnover and diet-tissue fractionation. The diet-muscle fractionations prior to the diet-switch were estimated to be −44.0‰, +1.9‰ and 0‰ for H, C and S, respectively, while the drinking water was demonstrated to be the main source of muscle O and thus δ18O variation. The diet-intra-muscular lipid fractionations prior to the diet-switch were estimated to be −172.7‰, −1.3‰ and −11.5‰ for H, C and O, respectively. The C half-lives of muscle were determined to be 75.7 and 91.6 days for animals receiving the high and low EA, respectively. Extracting temporally resolved pre-slaughter dietary information from meat by analysing bulk muscle, muscle lipids and muscle lipid fractions appeared to be not practicable due to possible incomplete turnover of lipids. |
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Keywords: | Stable isotopes Carbon Sulphur Hydrogen Oxygen Meat Muscle Lipid Lamb IRMS Isotopic clock |
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